Prazosin Protects the Liver Against Renal
                    Ischemia/Reperfusion Injury in Rats

Fatemeh Khajepour; Fariba Mahmoodpoor; Elmira Jafari; Farzad Kakaei; Farina Bahraminia; Shadi Aghajani; Sepideh Zununi Vahed; Yasin Bagheri

doi:10.1055/a-2015-7976

Drug Research, Table of Contents

Drug Res (Stuttg) 2023; 73(05): 289-295
DOI: 10.1055/a-2015-7976

Original Article

Prazosin Protects the Liver Against Renal Ischemia/Reperfusion Injury in Rats

Authors

Fatemeh Khajepour^*

¹Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran
Fariba Mahmoodpoor^*

²Kidney Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

³Department of Persian Medicine, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
Elmira Jafari

¹Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran
Farzad Kakaei

⁴Department of General and Vascular Surgery, Tabriz University of Medical Sciences, Tabriz, Iran
Farina Bahraminia

¹Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran
Shadi Aghajani

¹Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran
Sepideh Zununi Vahed

²Kidney Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Yasin Bagheri

²Kidney Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

Abstract

Acute kidney injury (AKI) is a common subsequent problem after many medical conditions. AKI is associated with distant organ dysfunction where systemic inflammation and oxidative stress play major roles. In this study, the effect of Prazosin, an α1-Adrenergic receptor antagonist, was investigated on the liver injury induced by kidney ischemia-reperfusion (I/R) in rats. Male adult Wistar rats (n=21) were divided into three groups: sham, kidney I/R, and kidney I/R pre-treated with Prazosin (1 mg/kg). Kidney I/R was induced by vascular clamping of the left kidney for 45 min to reduce the blood flow. Oxidative and antioxidant factors along with apoptotic (Bax, Bcl-2, caspase3), and inflammatory (NF-κβ, IL-1β, and IL-6) factors were measured in the liver at protein levels. Prazosin could reserve liver function (p<0.01) and increase glutathione level (p<0.05) after kidney I/R significantly. Malonil dialdehyde (MDA), a lipid peroxidation marker, was diminished more significantly in Prazosin-treated rats compared to the kidney I/R group (p<0.001). Inflammatory and apoptotic factors were diminished by Prazosin pre-treatment in the liver tissue (p<0.05). Pre-administration of Prazosin could preserve liver function and decrease its inflammatory and apoptotic factors under kidney I/R conditions.

Key words

acute kidney injury - prazosin - liver injury - distant organs

Full Text

References

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